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KMID : 0525720120170030157
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Journal of Chitin and Chitosan
2012 Volume.17 No. 3 p.157 ~ p.163
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Preparation and Effect of Methoxy Poly (ethylene glycol)-grafted-carboxymethyl Chitosan as a Doxorubicin Carrier
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Lee Kyoung-Jae
Nam Joung-Pyo
Park Seong-Cheol
Park Yung-Hoon
Nah Jae-Woon
Jang Mi-Kyeong
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Abstract
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A drug delivery system based on micelles or nanoparticles has attended due to the potential for long-circulation times, prevention of undesirable side effects, sustained release, and enhancing solubility of drug, Although doxorubicin (DOX), used in this study, is an anticancer drug in treatment of human malignancies, it has substantial risk of cardiomyopathy and lifethreatening heart failure. Therefore, we prepared methoxy poly (ethylene glycol) (PEG) and DOX-grafted O-carboxymethyl chitosan (OCMCh) used to reduce cytotoxicity and enhance treatment efficacy due to the enhanced permeability and retention effect. This self-aggregated nanoparticle was characterized by fourier transform infrared (FT-IR) and proton nuclear magnetic resonance (H NMR), dynamic light scattering (DSC), and transmission electron microscope (TEM), The results showed that the nanoparticles were spherical and their size were less than 400 nm. Its anti-tumor activity and cytotoxicity of DOX were investigated agaisnt MCF-7 and HEK293 cells in vitro, respectively, resulted in a significant increasing of anti-tumor activity upon Dox-grafting amounts and a reduced cytotoxicity compared to free Dox. It can be concluded that the OCMPEGDOX was a potential drug carrier.
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KEYWORD
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Carboxymethyl chitosan, Cancer, Doxorubicin, DDS, Poly, ethylene glycol
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